| Police |
| Byrnes Mill Police Department |
| Hillsboro Police Department |
| Jefferson County Animal Control |
| Jefferson County Emergency Management |
| Jefferson County Sheriffs Office |
| Kimmswick Police Department |
| Olympian Village Police Department |
Fire |
| Antonia Fire Protection District |
| Cedar Hill Fire Protection District |
| Desoto Rural Fire Protection District |
| Dunklin Fire Protection District |
| Goldman Fire Protection District |
| Hematite Fire Protection District |
| Herculaneum Fire Department |
| High Ridge Fire Protection District |
| Hillsboro Fire Protection District |
| Jefferson County Hazardous Material Team |
| Jefferson R-7 Fire Protection District |
| Mapaville Fire Protection District |
| Rock Community Fire Protection District |
| Shady Valley Fire Protection District |
| Springdale Fire Protection District |
EMS |
| Big River Ambulance District |
| North Jefferson County Ambulance District |
| Rock Township Ambulance District |
| Valle Ambulance District |